Malaysia is a rain forest country that has various types of wildlife. Urbanization activities like deforestation and plantation had significantly disturbed our ecology and biodiversity which leads to extinction problems. In November 2019, the last Sumatran Rhinoceros in Malaysia was died that triggered the awareness of Malaysian to concern about wildlife. The death of the last Sumatran Rhinoceros underscores how critically essential the collaborative efforts driving the preservation and conservation of the endangered species. From time to time, the number of the other endangered species are increasing. However, it is not too late to start the collection of samples such as tissue, gametes, and cells from the endangered species that still available in Malaysia and will be deposited in the established frozen zoo. At the same time, the researchers will start the new cellular technology namely induced pluripotent stem cells (IPSCs) or somatic cell nuclear transfer (SCNT) cloning approaches using the derived cell lines. Assisted Reproductive Technology (ART) could benefit conservation activities by focusing on breeding and establishing the genomic database. Somatic cell nuclear transfer (SCNT) and induced pluripotent stem cell (IPSC) technologies would be useful in conserving the endangered species at cell line level. This study will be serving the government and society by producing the viable embryo for the cloning activities in the future. This project will be divided into several phases which are deriving the cells of the endangered species; producing the viable embryo via SCNT approach; via iPSC technique; morphology, biochemistry, and genetic changes of the cryopreserved embryo. The findings from this study will serve as a guideline for the development of the endangered species’ embryo which will be ready for the breeding activities.